NIKON ECLIPSE Ni-L
Upright Microscopes for Research
The ECLIPSE Ni-L is Nikon's flagship upright microscope, boasting great optical performance and high system expandability. Combined with high-performance objectives, it can accurately visualize microstructure of the sample with high resolution. Adopting a stratum structure that allows simultaneous mounting of multiple fluorescence attachments, it provides great flexibility to freely combine various accessories as needed.
Manual model with built-in LED light source
- Manual focus
- Motorized nosepiece and motorized fluorescence cube turret available
- Built-in high color rendering LED light source
- Standard model suitable for brightfield observation of pathological specimens and digital imaging
- Supports a variety of activities, from research to routine examinations

Imaging of fluorescent-labeled specimens
In combination with a motorized nosepiece and a motorized epi-fluorescence cube turret, each unit is automatically controlled according to the camera settings such as exposure time, camera gain, and time interval, allowing fast, efficient image acquisition.
The motorized epi-fluorescence cube turret shutter, which helps to reduce photobleaching of specimens, is easily operated with a convenient Ni-L/Ni-U remote control pad.

CFI Plan Apochromat Lambda D series
The high-refractive index glass used in the oil-immersion objectives provides uniform brightness and high image quality up to the periphery of a large 25mm diagonal field of view, enabling efficient acquisition of seamless stitched images and supports macro imaging of large samples. High transmittance and chromatic aberration correction over a wide wavelength range from 405 nm to 850 nm enable reliable quantitative data acquisition for intensity analysis of nuclear staining. These high NA lenses are ideal for brightfield and DIC observations, as well as fluorescence and confocal observations.

Water dipping objective lenses
CFI Apochromat NIR 40X W/60X W objectives with long working distances and high NA provide high transmission over the near-IR wavelength range. Axial chromatic aberration is corrected up to the near-IR range, enabling high-resolution images of minute structures of thick samples during IR-DIC observations.
CFI75 Apochromat 25XC W and CFI Plan Achromat100XC W objectives featuring high NA (1.10) and long working distance (2.00 mm at 25XCW, 2.50 mm at 100XCW) are corrected for chromatic aberration in the IR range. These objectives can capture crisp images of deep regions of thick samples by adopting a mechanism to compensate for changes in spherical aberration that occur at different temperatures and observation point depths.

Uniformly bright illumination
A built-in “fly-eye” lens ensures uniform and bright illumination of the field of view, from edge-to-edge, at any magnification.

Fluorescence noise elimination
Nikon's proprietary noise terminator mechanism is employed in the epi-fluorescence cube turret and filter cubes. The S/N ratio is dramatically improved by eliminating stray light in the filter cubes, allowing images with weak fluorescent signals to be captured with high contrast and brightness.

High color rendering LED light source
The high color rendering LED light source built-into the Ni-L provides natural color reproducibility comparable to a halogen light source, as well as light uniformity, long life, and other advantages associated with LEDs, making it effective for observation of pathological specimens.

Simple digital imaging
Images can be captured with Digital Sight cameras by simply pressing the image capture button located on the microscope base.

Remote controller
The simple remote control pad for Ni-L/Ni-U makes it easy to control the motorized accessories. An ergo-controller with similar operational feel to the actual microscope is also available for Ni-E, facilitating intuitive operation.

Ergo controller Simple remote control pad
Compact lamphouse-free design
The Ni-L has a built-in LED light source for diascopic illumination, resulting in a space-saving design whose depth is approximately 150 mm shorter than that of the Ni-U, contributing to effective workspace utilization.

|
Ni-L |
Optical system |
CFI60 infinity optical system |
Focusing
(stroke from focus point) |
Via manual stage Up/Down movement
(Up 3 mm/Down 26 mm) |
Coaxial Coarse/Fine focusing |
Illumination |
Built-in high color rendering LED light source*1: 50,000 hours of life*2 |
Built-in fly-eye lens |
Controls |
Transmitted light On/Off switch, Intensity control dial with preset function
Image capture button |
· NI-SRCP Simple Remote Control Pad (option)
|
Power supply unit |
Built-in for LED illumination
NI-CTLB Control Box B is necessary when Motorized/Intelligent options are combined. |
Eyepieces (F.O.V. mm) |
· CFI 10X (22)
· CFI 12.5X (16)
· CFI 15X (14.5)
· CFI UW10X (25)
|
Tubes
F.O.V. 22 mm
(Eyepiece/Port) |
· C-TB Binocular Tube
· C-TE2 Ergonomic Binocular Tube (100/0, 50/50 via optional C-TEP2
DSC Port, C-TEP3 DSC Port C-0.55X or C-TEPF2.5 DSC Port F2.5X), Inclination angle: 10°-30°,
Extension up to 40 mm |
Tubes
F.O.V. 25 mm*3
(Eyepiece/Port) |
· C-TF Trinocular Tube F (100/0, 0/100)
· C-TT Trinocular Tube T (100/0, 20/80, 0/100)
· NI-TT2 Quadrocular Tilting Tube (Eyepiece/Upper port/Rear port: 100/0/0, 0/100/0, 0/0/100), Inclination angle: 15°-35°
|
Ports (F.O.V. 11 mm) |
· C-TEP2 DSC Port for Ergonomic Binocular Tube (with C-mount adapter, 0.7X)
· C-TEP3 DSC Port C-0.55X for Ergonomic Binocular Tube (with C-mount adapter, 0.55X)
· C-TEPF2.5 DSC Port F2.5X for Ergonomic Binocular Tube (with F-mount adapter, 2.5X)
· NI-BPU Back Port Unit (with C-mount adapter, 1.0X)
· NI-RPZ DSC Zooming Port for Quadrocular Tube (with C-mount adapter, manual zoom, 0.6X - 2.0X)
|
· NI-RPZ-E Motorized DSC Zooming Port for Quadrocular Tube (with C-mount adapter, motorized zoom, 0.6X - 2.0X) |
— |
Arms |
· NI-SAM Standard Arm
|
· NIU-CAM Contact Arm (for Motorized/Intelligent options)
|
Nosepieces
(Intelligent) |
· NI-N7-I Intelligent Septuple Nosepiece
|
Nosepieces
(Manual) |
· D-ND6 DIC Sextuple Nosepiece
· C-N6 ESD Sextuple Nosepiece ESD
· C-N6A Sextuple Nosepiece with Analyzer Slot |
Stages |
· C-CSR1S Right Handle Ceramic-coated Stage with 1S Holder
· C-CSR Right Handle Ceramic-coated Stage
· NIU-CSRR2 Right Handle Rotatable Ceramic-coated Stage with 2S Holder
Cross travel 78(X) x 54(Y) mm
Handle height and torque adjustable |
Substages |
· NI-SSR Substage (for Rotatable stage)
· NI-SS Substage (for Non-rotatable stages) |
Condensers
(Manual) |
· NI-CUD Universal Condenser Dry (0.88)
· C-AB Abbe Condenser (0.90)
· C-AR Achromat Condenser (0.80)
· C-DO Darkfield Condenser Oil (1.20-1.43)
· C-DD Darkfield Condenser Dry (0.80 - 0.95)
· C-AA Achromat Aplanatic Condenser (1.40)
· C-SA Slide Achromat Condenser 2-100X (0.90)
· C-SW Swing-out Achromat Condenser 1-100X (0.90/0.11)
· C-PH Phase Contrast Turret Condenser (0.90)*4
· C-SWA Swing-out Achromat Condenser 2-100X (0.90/0.22)
· C-LAR LWD Achromat Condenser (0.65)
· D-CUO DIC Condenser Oil (1.40)
|
Power consumption |
Main body: 28W (with max. high color rendering LED intensity)
Control Box B: 68W (with full motorized options) |
Weight (approx.) |
18 kg
(Brightfield configuration with ergonomic binocular tube) |
Epi-fluorescence
illuminator |
Ni-L |
Filter cube turret |
6 filter cubes mountable, Noise Terminator mechanism for all turrets
· NI-FLT6-E Motorized Epi-fluorescence Cube Turret
Motorized shutter, Status check function*5
· NI-FLT6-I IntelligentEpi-fluorescence Cube Turret
Manual shutter, Status check function*5
· NI-FLT6 Epi-fluorescence Cube Turret
Manual shutter |
Light distribution device |
· NI-FLEI-2 Epi-fluorescence Attachment
Aperture diaphragm and field diaphragm (Centerable/Detachable)
|
Option |
· NL-SC Shutter Cassette for Ni-L
|
Epi-illumination light source |
· D-LEDI Fluorescence LED Illumination system
|
*1 Cannot be used for IR-DIC observation.
*2 Estimate value based on Nikon’s regulations.
*3 Eyepiece F.O.V.: When used with an expanded configuration such as a double layer of fluorescent cube turrets, eyepiece F.O.V. is 22 mm. F.O.V. to imaging ports vary depending on the model.
*4 Can only be mounted on the NI-SS Substage.
*5 Status check function: Status of Filter/Nosepiece etc. can be recorded with captured images. Can be displayed on the PC operation screen.