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NIKON ECLIPSE Ni-U Research Upright Microscopes

The ECLIPSE Ni-U series is Nikon's flagship upright microscope, boasting great optical performance and high system expandability. Combined with high-performance objectives, it can accurately visualize microstructure of the sample with high resolution. Adopting a stratum structure that allows simultaneous mounting of multiple fluorescence attachments, it provides great flexibility to freely combine various accessories as needed.

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Overview Specifications

NIKON ECLIPSE Ni-U

Upright Microscopes for Research

The ECLIPSE Ni-U is Nikon's flagship upright microscope, boasting great optical performance and high system expandability. Combined with high-performance objectives, it can accurately visualize microstructure of the sample with high resolution. Adopting a stratum structure that allows simultaneous mounting of multiple fluorescence attachments, it provides great flexibility to freely combine various accessories as needed.

Manual model using halogen light source

Manual focus
Motorized nosepiece and motorized fluorescence cube turret available
Uses halogen light source
Standard model suitable for brightfield observation of pathological specimens and digital imaging
Supports a variety of activities, from research to routine examinations

ECLIPSE Ni-U

Imaging of fluorescent-labeled specimens

In combination with a motorized nosepiece and a motorized epi-fluorescence cube turret, each unit is automatically controlled according to the camera settings such as exposure time, camera gain, and time interval, allowing fast, efficient image acquisition.

The motorized epi-fluorescence cube turret shutter, which helps to reduce photobleaching of specimens, is easily operated with a convenient Ni-L/Ni-U remote control pad.

荧光标记标本的成像

CFI Plan Apochromat Lambda D series

The high-refractive index glass used in the oil-immersion objectives provides uniform brightness and high image quality up to the periphery of a large 25mm diagonal field of view, enabling efficient acquisition of seamless stitched images and supports macro imaging of large samples. High transmittance and chromatic aberration correction over a wide wavelength range from 405 nm to 850 nm enable reliable quantitative data acquisition for intensity analysis of nuclear staining. These high NA lenses are ideal for brightfield and DIC observations, as well as fluorescence and confocal observations.

CFI Plan Apochromat Lambda D series

Water dipping objective lenses

CFI Apochromat NIR 40X W/60X W objectives with long working distances and high NA provide high transmission over the near-IR wavelength range. Axial chromatic aberration is corrected up to the near-IR range, enabling high-resolution images of minute structures of thick samples during IR-DIC observations.

CFI75 Apochromat 25XC W and CFI Plan Achromat100XC W objectives featuring high NA (1.10) and long working distance (2.00 mm at 25XCW, 2.50 mm at 100XCW) are corrected for chromatic aberration in the IR range. These objectives can capture crisp images of deep regions of thick samples by adopting a mechanism to compensate for changes in spherical aberration that occur at different temperatures and observation point depths.

Water dipping objective lenses

Uniformly bright illumination

A built-in “fly-eye” lens ensures uniform and bright illumination of the field of view, from edge-to-edge, at any magnification.

A built-in “fly-eye” lens ensures uniform

Fluorescence noise elimination

Nikon's proprietary noise terminator mechanism is employed in the epi-fluorescence cube turret and filter cubes. The S/N ratio is dramatically improved by eliminating stray light in the filter cubes, allowing images with weak fluorescent signals to be captured with high contrast and brightness.

Fluorescence noise elimination

Ease of operation

The ergonomic tube and stage handle height adjustment mechanisms allow comfortable viewing positions. The space-saving microscope base enables users to maximize their work area. A simple remote control pad for easy operation of motorized accessories is also available.

Simple digital imaging

Images can be captured with Digital Sight cameras by simply pressing the image capture button located on the microscope base.

Remote controller

The simple remote control pad for Ni-L/Ni-U makes it easy to control the motorized accessories. An ergo-controller with similar operational feel to the actual microscope is also available for Ni-E, facilitating intuitive operation.

Ergo controller 简易遥控器

Ergo controller Simple remote control pad

	Ni-U
Optical system	CFI60 infinity optical system
Focusing (stroke from focus point)	Via manual stage Up/Down movement (Up 3 mm/Down 26 mm)
Coaxial Coarse/Fine focusing
Illumination	Halogen light source (12V100W)
Built-in fly-eye lens Built-in NCB11, ND8, ND32 filters (detachable, one additional filter mountable) and diffuser (non-detachable) ND2 filter (option)
Controls	Transmitted light On/Off switch, Intensity control dial with preset function Image capture button
· NI-SRCP Simple Remote Control Pad (option)
Power supply unit	Built-in for halogen illumination NI-CTLB Control Box B is necessary when Motorized/Intelligent options are combined.
Eyepieces (F.O.V. mm)	· CFI 10X (22) · CFI 12.5X (16) · CFI 15X (14.5) · CFI UW10X (25)
Tubes F.O.V. 22 mm (Eyepiece/Port)	· C-TB Binocular Tube · C-TE2 Ergonomic Binocular Tube (100/0, 50/50 via optional C-TEP2 DSC Port, C-TEP3 DSC Port C-0.55X or C-TEPF2.5 DSC Port F2.5X), Inclination angle: 10°-30°, Extension up to 40 mm
Tubes F.O.V. 25 mm^*3 (Eyepiece/Port)	· C-TF Trinocular Tube F (100/0, 0/100) · C-TT Trinocular Tube T (100/0, 20/80, 0/100) · NI-TT2 Quadrocular Tilting Tube (Eyepiece/Upper port/Rear port: 100/0/0, 0/100/0, 0/0/100), Inclination angle: 15°-35°
Ports (F.O.V. 11 mm)	· C-TEP2 DSC Port for Ergonomic Binocular Tube (with C-mount adapter, 0.7X) · C-TEP3 DSC Port C-0.55X for Ergonomic Binocular Tube (with C-mount adapter, 0.55X) · C-TEPF2.5 DSC Port F2.5X for Ergonomic Binocular Tube (with F-mount adapter, 2.5X) · NI-BPU Back Port Unit (with C-mount adapter, 1.0X) · NI-RPZ DSC Zooming Port for Quadrocular Tube (with C-mount adapter, manual zoom, 0.6X - 2.0X)
Arms	· NI-SAM Standard Arm
· NIU-CAM Contact Arm (for Motorized/Intelligent options)
Nosepieces (Motorized)	· NI-N7-E Motorized Septuple Nosepiece · NI-ND6-E Motorized DIC Sextuple Nosepiece
Nosepieces (Intelligent)	· NI-N7-I Intelligent Septuple Nosepiece
Nosepieces (Manual)	· D-ND6 DIC Sextuple Nosepiece · C-N6 ESD Sextuple Nosepiece ESD · C-N6A Sextuple Nosepiece with Analyzer Slot
Stages	· C-CSR1S Right Handle Ceramic-coated Stage with 1S Holder · C-CSR Right Handle Ceramic-coated Stage · NIU-CSRR2 Right Handle Rotatable Ceramic-coated Stage with 2S Holder Cross travel 78(X) x 54(Y) mm Handle height and torque adjustable
Substages	· NI-SSR Substage (for Rotatable stage) · NI-SS Substage (for Non-rotatable stages)
Condensers (Manual)	· NI-CUD Universal Condenser Dry (0.88) · C-AB Abbe Condenser (0.90) · C-AR Achromat Condenser (0.80) · C-DO Darkfield Condenser Oil (1.20-1.43) · C-DD Darkfield Condenser Dry (0.80 - 0.95) · C-AA Achromat Aplanatic Condenser (1.40) · C-SA Slide Achromat Condenser 2-100X (0.90) · C-SW Swing-out Achromat Condenser 1-100X (0.90/0.11) · C-PH Phase Contrast Turret Condenser (0.90)^*4 · C-SWA Swing-out Achromat Condenser 2-100X (0.90/0.22) · C-LAR LWD Achromat Condenser (0.65) · D-CUO DIC Condenser Oil (1.40)
Power consumption	Main body: 133W (with max. halogen lamp intensity) Control Box B: 29W (with full motorized options)
Weight (approx.)	20 kg (Brightfield configuration with ergonomic binocular tube)
Epi-fluorescence illuminator	Ni-U
Filter cube turret	6 filter cubes mountable, Noise Terminator mechanism for all turrets · NI-FLT6-E Motorized Epi-fluorescence Cube Turret Motorized shutter, Status check function^5 · NI-FLT6-I IntelligentEpi-fluorescence Cube Turret Manual shutter, Status check function^5 · NI-FLT6 Epi-fluorescence Cube Turret Manual shutter
Light distribution device	· NI-FLEI-2 Epi-fluorescence Attachment Aperture diaphragm and field diaphragm (Centerable/Detachable)
Option	· NL-SC Shutter Cassette for Ni-L
Epi-illumination light source	· D-LEDI Fluorescence LED Illumination system